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. 2009 Nov 15;20(22):4673–4685. doi: 10.1091/mbc.E09-02-0172

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© 2009 by The American Society for Cell Biology

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Figure 3. — Growth of the daughter cell is tightly blocked in class A mutants. Wild-type (SEC1) and the indicated sec1 and sec10 mutant cells were synchronized using α-factor. Growth of the daughter cell (bud) was initiated at restrictive temperature (38°C) by washing out the α-factor with fresh media. Images were taken at 0 and 60 min, by using DIC microscopy and analyzed using IP Lab Image Analysis software (BioVision Technologies). The percentage of small-budded cells (arrowheads; <2 μm; gray bars), large-budded cells (arrows; >2 μm; white bars), and unbudded cells (black bars) were quantified for the 60-min time point and plotted as a bar graph (n = 200).