Table 1.
Yeast strains
| Strain | Genotype | Source |
|---|---|---|
| CCY6 | BY4743, MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0 YDR164c::kanMx4/YDR164c (SEC1) | EUROSCARF |
| CCY32 | MATa leu2Δ0 his3Δ1 ura3Δ0 met15Δ0 YDR164c::KANMX pRS416 SEC1 | This study |
| CCY19 | CCY32, but pRS416 SEC1 is replaced with pCC64 SEC1 | This study |
| CCY28 | CCY32, but pRS416 SEC1 is replaced with pCC117 sec1-32 | This study |
| CCY29 | CCY32, but pRS416 SEC1 is replaced with pCC89 sec1-34 | This study |
| CCY44 | CCY32, but pRS416 SEC1 is replaced with pCC137 sec1-50 | This study |
| CCY45 | CCY32, but pRS416 SEC1 is replaced with pCC131 sec1-51 | This study |
| CCY46 | CCY32, but pRS416 SEC1 is replaced with pCC82 sec1-52 | This study |
| CCY50 | CCY32, but pRS416 SEC1 is replaced with pCC84 sec1-65 | This study |
| CCY51 | CCY32, but pRS416 SEC1 is replaced with pCC103 sec1-66 | This study |
| CCY52 | CCY32, but pRS416 SEC1 is replaced with pCC134 sec1-67 | This study |
| CCY53 | CCY32, but pRS416 SEC1 is replaced with pCC90 sec1-68 | This study |
| CCY55 | CCY32, but pRS416 SEC1 is replaced with pCC140 sec1-55 | This study |
| CCY57 | CCY32, but pRS416 SEC1 is replaced with pCC104 sec1-80 | This study |
| CCY58 | CCY32, but pRS416 SEC1 is replaced with pCC74 sec1-83 | This study |
| CCY59 | CCY32, but pRS416 SEC1 is replaced with pCC161 sec1-84 | This study |
| CCY64 | CCY32, but pRS416 SEC1 is replaced with pCC130 sec1-56 | This study |
| CCY65 | CCY32, but pRS416 SEC1 is replaced with pCC155 sec1-57 | This study |
| CCY66 | CCY32, but pRS416 SEC1 is replaced with pCC128 sec1-58 | This study |
| CCY67 | CCY32, but pRS416 SEC1 is replaced with pCC133 sec1-59 | This study |
| CCY71 | CCY32, but pRS416 SEC1 is replaced with pCC129 sec1-81 | This study |
| CCY91 | CCY32, but pRS416 SEC1 is replaced with pCC156 sec1-35 | This study |
| CCY92 | CCY32, but pRS416 SEC1 is replaced with pCC157 sec1-36 | This study |
| CCY94 | CCY32, but pRS416 SEC1 is replaced with pCC159 sec1-38 | This study |
| CCY95 | CCY32, but pRS416 SEC1 is replaced with pCC160 sec1-39 | This study |
| CCY100 | CCY32, but pRS416 SEC1 is replaced with pCC154 sec1-44 | This study |
| CCY102 | CCY32, but pRS416 SEC1 is replaced with pCC92 sec1-46 | This study |
| CCY104 | CCY32, but pRS416 SEC1 is replaced with pCC94 sec1-48 | This study |
| CCY105 | CCY32, but pRS416 SEC1 is replaced with pCC95 sec1-49 | This study |
| CCY106 | CCY32, but pRS416 SEC1 is replaced with pCC68 sec1-75 | This study |
| CCY107 | CCY32, but pRS416 SEC1 is replaced with pCC69 sec1-76 | This study |
| CCY108 | CCY32, but pRS416 SEC1 is replaced with pCC70 sec1-77 | This study |
| CCY109 | CCY32, but pRS416 SEC1 is replaced with pCC71 sec1-78 | This study |
| CCY110 | CCY32, but pRS416 SEC1 is replaced with pCC73 sec1-79 | This study |
| CCY162 | CCY32, but pRS416 SEC1 is replaced with pCC96 sec1-82 | This study |
| CCY42 | CCY32 sec18-1, product of a cross with NY432 (P. Novick) | This study |
| CCY43 | CCY42, but pRS416 SEC1 is replaced with pCC89 sec1-34 | This study |
| GY3323 | MATa leu2-3,112 ura3-52 trp1 his3Δ-200 exo70::HIS3 (CEN TRP1 exo70-38) L-A- | W. Guo |
| NY778 | MATα leu2-3,112 ura3-52 sec6-4 | P. Novick |
| NY784 | MATa, leu2-3,112 ura3-52 sec10-2 | P. Novick |