Table 2.

Mutagenesis of yeast SEC1

Mutagenesisa	Mutation in Sec1p	Growth defectb (°C)	Allele
Random	N81S, R638P	38	sec1-48
Random	F156S, I187N	38	sec1-46
Random	N167I, F221Y, F235L	38	sec1-35
Random	I249N	38	sec1-36
Random	S391Y, D429G, E446K, I544F	36	sec1-38
Random	M402T, Y437N, I478T	31	sec1-39
Random	L417S, L639S	38	sec1-32
Random	Y437N, I260T	35	sec1-49
Random	G443E, K494T	31	sec1-34
Random	F620S, R643I, L633V, G696S	38	sec1-44
Conserved	D251A	None	sec1-83
Conserved	D251A, R252A	None	sec1-84
Conserved	R252A	None	sec1-78
Conserved	R252A, D255A	28	sec1-51
Conserved	R252L, D255V, E604L	Lethal
Conserved	R252A, D307H	None	sec1-80
Conserved	R252A, D307H, H371D	36	sec1-68
Conserved	R252A, H371D	36	sec1-66
Conserved	R252A, E604A	None	sec1-81
Conserved	R252A, E604R	32	sec1-65
Conserved	R252E, E604R	Lethal
Conserved	R252L, E604L	38	sec1-55
Conserved	E604A	36	sec1-50
Conserved	D255A	37	sec1-52
Conserved	D255N	35	sec1-67
Conserved	D307H	None	sec1-75
Conserved	D307H, H371D	None	sec1-77
Conserved	H371D	None	sec1-76
Groove surface	K79E, Y80E, R169E, K170E	38	sec1-58
Furrow surface	G380R, E381K, D429K	None	sec1-59
Cleft surface	R63D	None	sec1-56
Cleft surface	K64E	None	sec1-82
Cleft surface	R63A, K64A	None	sec1-57
Cleft surface	R63D, K64E	Lethal
Cleft surface	E291K, E297R, D360R, D362K	None	sec1-79

^aRationale for mutagenesis.

^bTemperature at which growth defect is observed.