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KLP59D localizes to kinetochores and centrosomes. (A) Immunofluorescence of an anaphase S2 cell double-labeled for KLP59D and α-tubulin. Centrosomes denoted by yellow arrowheads. (B) High-magnification image showing the relative localizations of KLP59D, Cid, and α-tubulin in early anaphase. (C) Top, immunofluorescence image of a metaphase spindle double-labeled for KLP59D and γ-tubulin. Bottom panels, magnified images of one centrosome (inset in the top panel).
