Figure 8.

Model: KLP59D stimulates both anaphase Pacman- and Flux-based motility. KLP59D contributes indirectly to poleward Flux by binding and recruiting KLP10A to the spindle poles via the centrosomes. We speculate that KLP10A is modified during its transit through the centrosome, enabling it to function optimally as a MT depolymerizer at the spindle pole. KLP59D directly depolymerizes kinetochore MT plus ends during anaphase, thereby driving Pacman motility. In S2 cells centromeric KLP59C corrects aberrant chromosome attachments.