Figure 3.

RNAi of TbSec23.2 or TbSec24.1 delays VSG transport. Specific dsRNA synthesis was induced for 12 h in the TbSec RNAi cell lines as indicated, and the transport of newly synthesized VSG was assayed as in Figure 1D (3 × 10⁵ cell equivalents/lane). Phosphorimages of representative matched gels from control (Tet−) and silenced (Tet+) cells are presented (A–D, bottom). Loss of cell-associated VSG during hypotonic lysis was quantified and is presented as mean ± SEM for five independent experiments (A–D, top).