Figure 1.

Subcellular distribution of YY1 changes during mitosis. Indirect immunofluorescence of HeLa cells during mitosis. Endogenous YY1 (red) was immunostained with anti-YY1 antibody (H-414). The nuclear envelope was visualized by immunostaining of lamin A/C (green), followed by DAPI staining of DNA (blue). The different phases of mitosis were determined based on DNA conformation and nuclear envelope breakdown and reassembly (Mitchison and Salmon, 2001; Pines and Rieder, 2001; Watrin and Legagneux, 2003). Bar, 5 μm. Further analysis of telophase is presented in Supplementary Figure S1.