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. 2009 Nov 15;20(22):4766–4776. doi: 10.1091/mbc.E09-04-0264

Figure 4.

Figure 4.

Dephosphorylation of Flag-YY1 from nocodazole-arrested HeLa cells increases its DNA-binding activity. Flag-YY1, purified from nocodazole-arrested HeLa cells, stably transfected with pCS2(+)-Flag-YY1, was incubated 30°C, with or without λ-phosphatase for 30 min before use in the EMSA. The specificity of the YY1 shift was assessed by addition of anti-YY1 (C-20), anti-GFP, or anti-Sp1 to the reaction containing Flag-YY1 and λ-phosphatase, as indicated in A. Equal loading of Flag-YY1 in these reactions was assessed by Western blotting using anti-YY1 (H-10) antibody (B).