Figure 7.

Mutation of S247 has little effect on YY1 DNA-binding activity. HeLa cells were transiently transfected with pCS2(+)-Flag-YY1 WT, S247A, or S247D mutants. Cells that did not receive DNA served as negative control (Mock). Eighteen hours after transfection, WCEs were analyzed by Western blot using anti-YY1 antibody (H-10) (A), and EMSA using ³²P-labeled H3.2α as probe (B). Confocal imaging of Hela cells transfected with Flag-YY1 (WT) or S247 mutants is presented in Supplementary Figure S4.