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. 2009 Nov 15;20(22):4790–4803. doi: 10.1091/mbc.E09-07-0560

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© 2009 by The American Society for Cell Biology

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Figure 3. — Endogenous CENP-F and Hook2 proteins interact and colocalize at the centrosome. (A) Endogenous coimmunoprecipitation with C2C12 cells. Lysate samples were pulled down with CENP-F antibody and blotted with Hook2 antibody. The third column shows no Hook2 pulled down when no CENP-F antibody was used in the pulldown. (B–J) Endogenous antibodies to CENP-F, Hook2, and γ-tubulin were used in C2C12 cells to show colocalization of all three proteins. (B) γ-tubulin centrosome marker. (C) Hook2. (D) Merge with differential interference contrast (DIC). (E) γ-tubulin. (F) CENP-F. (G) Merge. (H) CENP-F. (I) Hook2. (J) Merge with DIC. Arrows show colocalization and arrowheads show additional protein localizations. Bars 10 μm.