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. 2009 Nov 5;8:29. doi: 10.1186/1476-0711-8-29

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Copyright © 2009 Ishida et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transmission electron micrographs of C. neoformans ATCC 28957 strain grown in the presence of subfraction F2.4 from S. adstringens for 72 h, at 35°C. Untreated yeasts have a compact cell wall (cw) surrounded by a polysaccharide capsule (c) (A); Yeasts treated with 1 μg/ml (B-D) and 2.5 μg/ml (E-F) show several ultrastructural alterations, such as cell-wall disruption (black arrow), presence of vacuoles (v) mitochondrial swelling (m) and many membranous structures in the cytoplasm (black arrowhead). Bars = 1 μm.