Table 3.
Adoptive transfer of CD4+ cells from tolerized mice at week 6 rendered partial dominant tolerance to sygeneic mice
| CD4+ cell donors | hFVIII activity of receivers, percentage of normal |
Anti-hFVIII antibodies of receivers, Bethesda units |
||||
|---|---|---|---|---|---|---|
| Day 7 | Day 14 | Day 21 | Day 7 | Day 14 | Day 21 | |
| Naive | 317.0 ± 76.2 | 2.2 ± 2.3 | 0.5 ± 0.6 | 0 | 0 | 28.5 ± 5 |
| FVIII plasmid only | 28.1 | 0 | 0 | 1.5 | 55 | 97 |
| FVIII plasmid + anti-CD3 (week 2) | 331.1 | 12.5 | 0 | 0 | 5 | 20 |
| FVIII plasmid + anti-CD3 (week 6) | 262.5 ± 9.3 | 95.2 ± 46.4 | 40.0 ± 39.7 | 0 | 0 | 0 |
Cells were isolated from donors 2 weeks or 6 weeks after plasmid injection.