Figure 7.

Response of Purkinje Cells to Bursts of CF Input

(A) Recording configuration for simultaneous whole-cell somatic and dendritic recordings from Purkinje cells in acute slices.

(B) Example dendritic (upper traces, recorded 85 μm from the soma) and somatic (lower traces) Purkinje cell responses to one, three, and five CF stimuli at 2 ms intervals (black triangles). Several superimposed traces (gray); single examples shown in blue (dendritic recording) and red (somatic recording). Dendritic and somatic data are shown with different timescales. Blue arrows, post-complex spike pause duration; asterisk, additional dendritic calcium spike.

(C) Mean (±SEM) number of dendritic spikes (n = 3 cells) in response to CF burst stimulation. Stimuli were given at intervals of 2 ms (dark blue markers), 3 ms (green markers), or patterns mimicking recorded olivary axon bursts (light blue markers).

(D) Mean (±SEM, n = 10) number of somatic spikes in response to CF burst stimulation, protocol as in (B). Red line marks unity, where Purkinje cell spike number is equal to number of CF stimuli.

(E) Mean (±SEM, n = 7) number of CF burst-evoked spikes predicted to be propagated by the Purkinje cell axon (see Experimental Procedures). Stimulation protocol as in (B), red line as in (D).

(F) Average (mean ± SEM) post-complex spike pause length following bursts of CF stimulation. Responses in which a burst of CF stimuli elicited additional dendritic spikes are averaged separately and marked with an asterisk.