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Myelination increases with time in culture. Co-labelled images stained for proteolipid protein (PLP)–DM20 (A, C, E and G) and axonal phosphorylated neurofilament (B, D, F and H) at 20 days (A and B), 23 days (C and D) and 26 (E and F) or 40 days (G and H) days in vitro. All time frames were harvested from the same culture. Scale bar: 200 μm.
