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. 2008 Oct;28(8):1518–1535. doi: 10.1111/j.1460-9568.2008.06415.x

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Journal compilation © 2008 Federation of European Neuroscience Societies and Blackwell Publishing Ltd

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Fig. 8 — Examples of cultures used for biochemical preparations. Biochemical preparations were obtained from myelinating cultures derived from embryonic mice and grown for 33 days (A and B) and from dissociated neonatal mouse spinal cord oligodendrocytes, cultured for 7 days (C and D). Myelinating cultures were co-labelled for proteolipid protein (PLP)–DM20 (A) and phosphorylated neurofilament (B). Mouse oligodendroglia were co-stained for cell surface PLP–DM20 using the O10 antibody on live cells (C), and for cytosolic PLP–DM20 using AA3 antibody (D) after fixation and permeabilisation of cells. Scale bars: A and B, 200 μm; C, 50 μm.