Table 3.
Effects of 5-HT2C receptor antagonist and inverse agonist on wheel-running behaviour in C2CR mice
| Treatment and time of day | Control (revolutions) | C2CR (revolutions) |
|---|---|---|
| Basal | ||
| 19:00–20:00 h | 5038 ± 318 | 3228 ± 912* |
| 20:00–21:00 h | 4405 ± 657 | 2379 ± 842 |
| Vehicle | ||
| 19:00–20:00 h | 4462 ± 420 | 1523 ± 1050* |
| 20:00–21:00 h | 3791 ± 971 | 2603 ± 844 |
| Antagonist SB242084 (3 mg/kg) | ||
| 19:00–20:00 h | 3751 ± 916 | 2093 ± 784* |
| 20:00–21:00 h | 3445 ± 1054 | 1671 ± 772 |
| Inverse agonist SB206553 (1 mg/kg) | ||
| 19:00–20:00 h | 3149 ± 1175 | 1907 ± 681 |
| 20:00–21:00 h | 3794 ± 913 | 1254 ± 570 |
| Inverse agonist SB206553 (5 mg/kg) | ||
| 19:00–20:00 h | 1401 ± 440† | 351 ± 325*,† |
| 20:00–21:00 h | 2074 ± 713 | 1097 ± 687 |
The number of wheel revolutions generated by the control and C2CR mice in the 2 h subsequent to the lights being switched off were counted in untreated mice (basal) and treated mice: 30 min following the administration of vehicle, antagonist (SB242084; 3 mg/kg) or inverse agonist (SB206553; 1 or 5 mg/kg). While the C2CR mice generally carried out less wheel running than the control mice (F1.50 = 13.0, P < 0.001), there were differences between the treatment groups (F4.50=4.9, P < 0.01). However, the effect of the drug treatment was similar across the genotypes (i.e. no interaction). Data are mean ± SEM; n = 6; *P < 0.05 compared with the respective control mice; †P < 0.05 compared with the respective saline group.