Figure 4.

S27D induces diffused localization of alpha-catenin. Endothelial cells were cultured and grown to confluency on FN-coated glass cover slips and subsequently incubated with the control peptide (CTRL; upper panel) or S27D (lower panels) for 30 minutes. Next, cells were fixed, permeabilized and stained with streptavidin to detect S27D (green) and Abs to beta-catenin, VE-cadherin, alpha-catenin and p120-catenin (red). Merge shows the co-localization of S27D and the indicated proteins in yellow (Arrowheads). Bar, 20μm.