Figure 5.
AVV-PRS-hKir2.1 does not increase allodynia in spared nerve injury model. A, To assess the effect of transduction with AVV-PRS-hKir2.1 on the development of the signs of neuropathic pain, animals received lumbo-spinal injection of either AVV-PRS-hKir2.1 or AVV-PRS-EGFP 1 week before SNI. Each group was then followed for 2 weeks and assessed for the development of mechanical and cold allodynia. B, C, After spinal AVV administration, both groups of SNI animals developed significant (B) mechanical allodynia (von Frey hair threshold) and (C) cold allodynia (acetone drop induced paw withdrawal). Transduction of the pontospinal NAergic neurons with AVV-PRS-hKir2.1 did not significantly alter the expression of mechanical or cold allodynia compared with AVV-PRS-EGFP (two-way rmANOVA).