Figure 4.

DNA binding of TraA-tag using the DPAC method. Extracts containing overexpressed TraA-tag and RsaI- or DraI-digested pAM7500 were used in the DPAC procedure as described in the Materials and Methods. Eluted material was extracted with phenol-chloroform, submitted to agarose gel electrophoresis, and stained with ethidium bromide. The restriction fragment “held” to the matrix in the case of RsaI is seen in lane A; the fragment held in the case of DraI is seen in lane B. The lane labeled 1 kb represents marker DNA (1-kb ladder).