Figure 3. Measurement of MSP1₄₂ and free anti-MSP1 antibodies in maternal and fetal circulation with the placental perfusion model.

The levels of MSP1 in the fetal perfusate were measured before (nondissociated) and after treatment with dissociation buffer (dissociated) during the 3 phases of the experiment. The differences in levels of MSP1₄₂ detected in the fetal perfusate between phases II and III (placentas 3 and 5) correlated with percentage of plasma containing anti-MSP1 antibodies added to the maternal compartment. Each point represents the mean value performed in triplicate. The variation between replicate samples was <10%. Assay 1 was used to measure free MSP1 and assay 2 for free anti-MSP1 antibodies (see table 1 ).