Figure 2. Huia sexing using semi-nested PCR with CHD primers p2/p5 followed by p2/p3.

Amplification was followed by digestion of the Z CHD fragment with the restriction enzyme HaeIII. The W chromosome CHD fragment in Huia is 121 bp long and the Z chromosome fragments are 65 and 56 bp in length, after enzyme digestion. Known-sex relatives of Huia, the North Island Kokako and Saddleback, were used to verify the test. Molecular weight maker sizes at shown at left.