Figure 3. In situ detection of S-nitrosylated targets using different nitric oxide donors.

Spermatozoa were incubated with GSH (top), spermine NONOate (middle) or GSNO (bottom) for 1 hr prior S-nitrosylated proteins labelling with Texas-red fluorescence-MTSEA as described in Materials and Methods. Fluorescence images were obtained using using 100X objective. Results of 1 experiment presented are representative of 3 others performed with different sperm donors.