Figure 1.

Human Hsp90α and yeast Hsc82 in solution have an extended conformation distinct from the bacterial HtpG structure. (A) Two previously described conformations of apo HtpG.^14,16 (B) Comparison of the P(r) functions for apo Hsc82 (black) and apo Hsp90α (purple). (C) Hsc82 (black) and Hsp90α (purple) have a larger D_max than either known apo bacterial Hsp90 solution state [high pH,¹⁶ blue; low pH (Krukenberg et al., in press), orange]. The human and yeast proteins also display a distinctive shape to their P(r) curves.