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Reaction of wild-type ALAS, R85K, R85L, and R85L/T430V (5 μM) with ALA. The observed rate constants were calculated by fitting the decrease in intrinsic protein fluorescence over time to Eq. (2) for a single exponential process. (A) wild-type ALAS; (B) R85K; (C) R85L; and (D) R85L/T430V.
