Table I.

Residues Tested for Constraint by Ligand Binding

	GBP		OmpA
	pa	mb	p	TMc
Strongd
Q14	+	+	+	+
Q33	+	+	+	+
K41	+	+	+	+
L42	+	+	+	+
D43	+	+	+	+
D45	+	+	+	+
T65	+	+	+	+
S85	+	+	+	+
A87	+	+	+	+
L126	+		+
N127	+		+
P130	+		+
Significantd
T10	+		+
D20	+		+
E24	+		+
K34	+	+	+	+
D35	+		+
T46	+	+	+	*
T63	+		+
S67	+		+
E77	+	+	+	+
Q79	+		+
T92	+	+	+	+
C97	+		+
L136	+	+	+	*
L141	+	+	+	+
Q144	+		+
No Changed
S2	*	*	*	*
M9	*		*
Q50	*	*	*	*
V59	*	*	*	*
L75	*	*	*	*
N104	*		*
Q143	*	*	*	*
G146	*	*	*	*
E147	*		*	*
G148	*		*
T149	*		*
E150	*		*
A155	*		*

A change in spectral line shape observed that is consistent with the category is indicated with a plus symbol (+). An asterisk (*) indicates no change in spectral line shape was observed. The data included for precursor galactose-binding protein were previously published.¹³

^ap indicates the precursor form of either galactose-binding protein (GBP) or OmpA.

^bm indicates the mature form of galactose-binding protein (GBP).

^cTM indicates the truncated OmpA, OmpA-TM.

^dThe residues are divided according to the degree of constraint.