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Enrichment of bacteria displaying peptides with varying specificity for the target antibody. Cells from the unsorted (A), cycle 1 sort (gate red++green−) (B), and cycle 4 sort (gate red++green−−) (C) populations of the random library. Cells from the unsorted (D) and round 4 sort for specificity (gate red++green−−) (E) and crossreactivity (gate red−green++) (F) of the linear library. Cells were incubated with 1 μM disease IgG and either ×10 (A, B, D) or ×100 (C, E, F) control IgG.
