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Midpoints from urea-induced unfolding of C-terminal sequence variants. Substitutions of leucine to phenylalanine in the hydrophobic core in the background of full-length (left) and deletion construct N-15 (right), in LRR 15, in LRR 14, and in LRR 13. Conditions: 200 mM NaCl, 20 mM Tris-HCl (pH 7.6), and 0.01 mM TCEP.
