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Yields of Fusion Polypeptide and Cleaved Peptide from a 4 L of Shake-Flask Culture
| Family | Construct | Fusion protein yield (4 L) | Final yield (4 L) |
|---|---|---|---|
| Amylin | Amylin (carboxylate) | 130 mg | 9 mg |
| Amylin free acid | 250 mg | 10 mg | |
| Amylin + KR | 180 mg | 6 mg | |
| Amylin + CT | 170 mg | 7 mg | |
| Proamylin | 150 mg | 4 mg | |
| Gelsolin | 5 kDa aGel | 210 mg | 9 mg |
| 8 kDa aGel | 170 mg | 6 mg | |
| α-Synuclein | Major core | 220 mg | 12 mg |
| Minor core | 330 mg | 7 mg | |
| Insulin | Proinsulin | 206 mg | 10 mg |
| Akita proinsulin | 140 mg | 8 mg |
