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. 2009 Nov 26;5(11):e1000670. doi: 10.1371/journal.ppat.1000670

Figure 1. Time-dependent morphological changes in GcAVs.

Figure 1

HeLa cells expressing GFP-LC3 were incubated with GAS for 1 h and fixed at 0, 0.5, 1, 1.5, and 2 h after infection, followed by DAPI staining to visualize GAS. The percentage of GcAVs with a given morphological feature was quantified. The defined categories are as follows: (A) type A, a linear chain-like structure (B) type B, an aggregated structure with a clear GFP-LC3 boundary between each cocci; (C) type C, an aggregated structure with a partially discontinuous inner boundary; (D) type D, a semi-spherical structure without a clear inner boundary. Green, GFP-LC3; Magenta, DAPI. N, nuclear. Scale bars, 5 µm. (E) The values are expressed relative to the total number of GcAVs at each time point. Blue, Type A; Pink, typeB; Green, TypeC; Cyan, typeD. The results shown are representative of three independent experiments. Approximately 60 cells were analyzed per experimental group at each time point.