Figure 4. Response of a γ9-3 chimera.

A. C terminal sequences of relevant γ subtypes. B. Representative images and plots of γ9-3 chimera and PH-mCh translocation. Experiments and analysis were performed as described in Fig. 3A . C. Bar diagrams of the extent of PH-mCh translocation at different concentrations of agonist in cells expressing indicated γ subunits. Values were calculated as in Fig. 1B D. Ratio of the 100 µM over 1 µM response. Results are represented as mean ± SEM (N =  no γ-51, γ3-52, γ9-54, γ9-3 -40). **p<0.001 (γ9 versus others at the same concentration).