Figure 2. Strategy for Cre-mediated ablation of Wwox expression.

(A) To promote deletion of Wwox^flox alleles we used transgenic mice carrying the Cre-recombinase gene under control of the adenoviral EIIA-promoter. EIIA-regulated Cre-recombinase is expressed in pre-implantation embryos leading to site-specific deletion of LoxP flanked (floxed) sequences in all tissues including germ cells. (B) PCR-based strategy to demonstrate Cre-recombinase deletion of the Wwox target sequences. (C) Wwox protein expression is abolished in Wwox KO mice. Total protein extracts from the indicated tissues were analyzed by immunoblotting using Wwox specific antibodies. Anti-actin was used as a loading control. WT-wild-type, HET-heterozygous, KO-knockout.