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. 2000 Aug 29;97(18):10156–10161. doi: 10.1073/pnas.97.18.10156

Figure 2.

Figure 2

Glycosphingolipid Ag responses are affected by mutations at the bottom of the A′ pocket. (A) Structure of α-GalCer. AGL 587 is an α-GalCer analog with the 26-carbon acyl chain reduced to 2 carbons in length. (B) APCs expressing low levels of wild-type mCD1.1 (A20 wt CD1/8) or A′ pocket mutant mCD1.1 molecules were pulsed with 100 ng/ml either α-GalCer or AGL 587 before being cultured with indicated hybridomas. Cell-free supernatants were tested by ELISA for IL-2 content. One representative experiment of three is shown.