Fig. 2.

Myostatin (MSTN) regulates Akt phosphorylation in myotubes. Representative Western blot analysis of phospho-Akt (s473), total Akt, and GAPDH from myotube lysates. Quantification of Western blot results from 4 to 9 independent experiments is shown as percent maximal insulin-like growth factor-I (IGF-I) stimulation. Dividing lines on Western blot images depict where bands from the same blot have been juxtaposed. dnMSTN, dominant negative MSTN. *P < 0.001 vs. GFP, ‡P < 0.001 vs. GFP+IGF-I, #P < 0.05 vs. GFP.