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The 3′-strand of mivaRNAI is efficiently incorporated into RISC. The RISC associated RNAs from wild-type Ad5 infected cells were analyzed for the abundance of 3′-strand (A) and 5′-strand (B) of mivaRNA I by northern blotting. Different amounts (from 0.05 to 5 pmol) of chemically synthetic mivaRNAI were phosphorylated and used as standards for quantification.
