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Cleavage/religation equilibrium of the full duplex DNA substrate. Gel electrophoresis of the products coming from the incubation of the wild type topoisomerase I with the [γ-32P] end-labelled duplex DNA, shown at the top of the figure in the absence and presence of CPT and at different KCl concentrations. The arrow at the DNA sequence indicates the CL1 preferred cleavage site. Lane 1–4 (wild type), lane 5–8 (Lys681Ala). The asterisk indicates the band corresponding to the CL1 site.
