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. 2009 Sep 18;37(20):6849–6858. doi: 10.1093/nar/gkp669

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Suicide cleavage experiment for the wild type and Lys681Ala mutant. (A) Time course of the suicide cleavage reaction carried out with the substrate described on the top of the figure. CL1 and CL2 identify the cleaved complexes at the site indicated by the arrow and the asterisk respectively. Lane C, no protein added. (B) Percentage of the DNA substrate cleaved plotted at different time for the wild type (black line) and the Lys681Ala mutant (red line).