Table 2.
Abundance of TLS-assisted NHEJ events in various cell lines
| Cell line | TLS event | DNA sequence | Occurrence |
|---|---|---|---|
| PC3 | Full-length | 5′CAGGTCTGTT↓ATCCGGTATC | 2 |
| Skipping | 5′CAGGTCTG-T ATCCGGTATC | 5 | |
| Skipping | 5′CAGGTCTG-A ATCCGGTATC | 1 | |
| Skipping | 5′CAGGTCTG-C ATCCGGTATC | 1 | |
| N addition | 5′CAGGTCTGGGGATCCGGTATC | 1 | |
| Total TLS events: | 10/19 (53%) | ||
| NBS1+ | Full length | 5′CAGGTCTGCA↓ATCCGGTATC | 2 |
| Full length | 5′CAGGTCTGTT ATCCGGTATC | 1 | |
| Skipping | 5′CAGGTCTG-T ATCCGGTATC | 2 | |
| Total TLS events: | 5/29 (17%) | ||
| NBS1− | Full length | 5′CAGGTCTGTA↓ATCCGGTATC | 2 |
| Full length | 5′CAGGTCTGAT ATCCGGTATC | 1 | |
| Skipping | 5′CAGGTCTG-T ATCCGGTATC | 2 | |
| Total TLS events: | 5/24 (21%) | ||
| H1299a | Full length | 5′CAGGTCTGNM↓ATCCGGTATC | 38 |
| Skipping | 5′CAGGTCTG-T ATCCGGTATC | 79 | |
| Skipping | 5′CAGGTCTG-N ATCCGGTATC | 7 | |
| N addition | 5′CAGGTCTGGGGATCCGGTATC | 5 | |
| Total TLS events: | 129/232 (56%) |
NHEJ experiments were conducted with the indicated cell lines, after which descendants of plasmids with a cDSB were isolated and subjected to DNA sequence analysis.
aThe data for H1299 cells was taken from Figure 3, from sequence data obtained with pre-irradiated H1299 cells which were described in the text (data not shown). The detailed sequence changes in the 38 NHEJ events associated with full-length TLS (schematically presented as NM) are presented in Figure 3C and D.