Table 3.
Repair of complex DSB (substrate LP41) relative to simple DSB (substrate LP40) in hamster and human cells defective in NHEJ genes
| Cell type/vector | Number of colonies (DNA amounta) |
Relative repair b % | |
|---|---|---|---|
| KanR | CmR | ||
| CHO K1 (wild-type) | |||
| LP41 | 203 (350) | 253 (24) | 56.1 ± 7.0 |
| LP40 | 393 (350) | 260 (24) | |
| CHO XR1 (XrccIV−/−) | |||
| LP41 | 6 (350) | 636 (24) | NA |
| LP40 | 2 (350) | 364 (24) | |
| CHO XRS5 (Ku80−/−) | |||
| LP41 | 106 (200) | 1228 (24) | 51.0 ± 4.9 |
| LP40 | 203 (200) | 1122 (24) | |
| Human Guetel/DA4 (Artemis+) | |||
| LP41 | 106 (200) | 1072 (12) | 35.3 ± 9.6 |
| LP40 | 278 (200) | 784 (12) | |
| Human Guetel (Artemis–) | |||
| LP41 | 54 (200) | 1164 (12) | 18. 5 ± 3.4 |
| LP40 | 242 (200) | 1084 (12) | |
| Human 48BR (Artemis+) | |||
| LP41 | 28 (300) | 666 (24) | 15 ± 4.9 |
| LP40 | 59 (300) | 205 (24) | |
| Human CJ179 (Artemis–) | |||
| LP41 | 28 (300) | 237 (24) | 7 ± 2 |
| LP40 | 108 (300) | 80 (24) | |
NHEJ assays were performed with the indicated cell lines as described in the legend to Table 1 and under ‘Materials and Methods’ section. The colonies numbers shown represent a typical experiment, whereas the relative repair represents the average obtained from three to six experiments.
aThe numbers in the parentheses show the amount of DNA (in nanograms) used to transfect the mammalian cells.
bRelative repair of cDSB relative to sDSB.