Table 2.

Oligoduplexes used in this study^a

Oligoduplex	Sequence
T/2a	5′ CGCACGCCTTCCTGGAAGCACACTA 3′
	3′ GCGTGCGGAAGG2CCTTCGTGTGAT 5′
2/2a	5′ CGCACGCCTTCC2GGAAGCACACTA 3′
	3′ GCGTGCGGAAGG2CCTTCGTGTGAT 5′
2NSb	5′ CGCACGCCTTCCTGGAAGCACACTA 3′
	3′ GCGTGCGGA2GGACCTTCGTGTGAT 5′
2NS′b	5′ CGCACGCCTTCCTGGAAGCACACTA 3′
	3′ GCGTGCGG2AGGACCTTCGTGTGAT 5′
T/A	5′ CGCACGCCTTCCTGGAAGCACACTA 3′
	3′ GCGTGCGGAAGGACCTTCGTGTGAT 5′

^aOverlapping Ecl18kI/EcoRII-C/PspGI/PfoI recognition site is in boldface; 2 = 2AP, is indicated in boldface and underlined.

^bDuplexes 2NS and 2NS′ contain 2AP introduced immediately adjacent to the target site. The 2NS duplex was used in the Ecl18kI/EcoRII-C/PspGI experiment and the 2NS′ duplex was used in the PfoI experiment.