Skip to main content
. 2009 Sep 9;37(20):6701–6715. doi: 10.1093/nar/gkp727

Figure 1.

Figure 1.

Construction and expression of methyltransferases targeted to the mitochondria. A DNA fragment coding for a mitochondria targeting sequence (MTS) derived from the COX VIII gene was added to the 5′-end of the DNA methyltransferase genes and a HA tag was added to the 3′-end of the genes. The upper panel shows co-localization of ApaI methyltransferase and mitotracker in human osteosarcoma cells transfected with mito-M.ApaI-HA. The lower panel shows a similar experiment performed in HEK293T cells transfected with mito-M.CviPI-HA.