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Initial rates of aminoacylation for H. pylori tRNAGlu1 (green), tRNAGlu2 (red) and tRNAGln (black) using H. pylori GluRS1 (white bars) and GluRS2 (black bars). GluRS2 preferentially aminoacylates tRNAGln, whereas GluRS1 aminoacylates tRNAGlu1 and tRNAGlu2. The aminoacylation rate is determined by measuring the rate of formation of glutamyl-tRNAs in pmoles per unit of GluRS1 or GluRS2. Error bars represent standard deviation from triplicate assays.
