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. 2009 Sep 15;37(20):6942–6949. doi: 10.1093/nar/gkp754

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Investigation of the role of the D-stem and D-loop. Transfer RNAs were constructed to contain only the tRNA^Glu2 D-stem (red) or D-Loop (red) in a tRNA^Gln (black) background. Neither mutant tRNA was a robust substrate for either GluRS1 or GluRS2. Data to the left of the dashed line are reproduced from Figures 1 and 2 for comparison. The white and black bars indicate data obtained from GluRS1 and GluRS2 assays, respectively. Error bars represent standard deviation from triplicate assays.