Figure 1.

Two-hybrid interactions of Rad51 with Srs2. (A) Fragments of Srs2 were tested for two-hybrid interaction with Rad51 by plating on medium lacking leucine, tryptophan and adenine. The empty vector (pGBKT7) and plasmid harboring RAD52 were included as negative and positive controls, respectively. (B) A schematic of the helicase and protein-protein interaction domains in Srs2. (C) Purified Srs2, srs2 1–998, srs2 1–910, srs2 1–860, srs2 1–890 and srs2 1–875, 1 µg each, were analyzed by SDS-PAGE and stained with Coomassie Blue.