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. 2009 Sep 10;37(20):6754–6764. doi: 10.1093/nar/gkp748

Figure 5.

Figure 5.

Anti-recombinase activity examined by DNA strand exchange. (A) The DNA strand exchange scheme. (B) In panel I, circular ϕX174 (+) strand DNA was incubated with Rad51, RPA, and in the absence or presence of Srs2, srs2 Δ875–902 or srs2 1–860 (30 and 60 nM), and then spermidine and linear ϕX dsDNA were added and the reaction incubated for either 40 or 80 min. A reaction without Srs2 or mutant srs2, designated as standard (Std; lane 2) served as a control. The DNA substrates were also incubated without any protein (Bl; lane 1). Only the results from the 80 min time-point are shown. In panel II, the sum of joint molecules and nicked circular duplex from the 40 min and 80 min time-points of reactions that contained 30 nM Srs2 or srs2 mutant was plotted. In all cases, error bars represent the standard deviations derived from the results of three independent experiments.