Fig. 5.

Effect of SST on the kinetics of butyrate uptake in Caco2 cells. Overnight serum-starved postconfluent Caco2 cells were treated with 50 nM SST in serum-free cell culture medium for 30 min. [¹⁴C]Butyrate uptake was measured for 5 min in the presence of increasing concentrations of the substrate, butyrate (0.5–15 mM). [¹⁴C]Butyrate uptake was expressed as nmol/mg protein per 5 min. Representative Michaelis-Menten plot of experiments performed on 3 different occasions is shown.