Fig. 7.

SST induces MCT1/CD147 association in Caco-2 cells. Overnight serum-starved postconfluent Caco2 cells were treated with SST (25 and 50 nM) in serum-free cell culture medium for 30–60 min. Cells were also pretreated with the p38 MAPK inhibitor SB203580 (30 μM) for 60 min and then coincubated with SST (25 nM) for another 30–60 min. Cells were washed with 1× PBS, lysed, and immunoprecipitated (IP) with anti-CD147 antibody. Immunoprecipitates were analyzed by 10% SDS-polyacrylamide gel electrophoresis, followed by transfer of proteins to nitrocellulose and probed with anti-MCT1 antibody (MCT1). The Western blots (WB) were stripped and reprobed with the anti-CD147 antibody (CD147) to indicate equal loading of protein in each lane (A). A representative blot of 3 different experiments is shown. The data were quantified by densitometric analysis and expressed as arbitrary units (A.U.) and represent means ± SE of 3 determinations (B). *P < 0.05 compared with untreated control.