Fig. 5.
Symmetrical replacement of bicarbonate or chloride increases JMSOx in short-circuited Caco-2 monolayers 6–8 days postseeding. Open bars, Con monolayers; shaded bars, cells transfected with SLC26A6 siRNA (A6KD). Stilbene sensitivity was assessed in Per II by the addition of 100 μM DIDS to the mucosal compartment, and the residual, DIDS-insensitive components are indicated by the dark portion at the base of each bar. In standard buffer (Std Buffer, n = 6), GT was 3.07 ± 0.12 for Con and 3.49 ± 0.25 for A6KD monolayers and in HCO3−-free buffers (n = 10) GT was not significantly changed. In Cl−-free buffer (n = 6), GT was 1.43 ± 0.06 for Con and 1.40 ± 0.08 for A6KD monolayers. *Significant difference between Con and A6KD in a given buffer; †significant difference from the appropriate standard control buffer as judged by a 1-way ANOVA. Error bars represent ±1 SE.