Fig. 10.
A: effect of G protein Abs on contraction induced by PGF2α (10−6 M) in normal permeable colon muscle cells. Abs against Gαi3 blocked the contraction induced by PGF2α (10−6 M) (*P < 0.01 by ANOVA), whereas Abs against Gαq/11, Gαi1/2, and Gαs had no effect. Values are means ± SE of n = 4. B: Gαs protein Ab treatment in normal permeable cells almost completely abolished the inhibition of contraction induced by PGE2 (10−6 M) compared with that induced in control muscle cells (#P < 0.005 by ANOVA). Values are means ± SE of n = 3.