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. 2009 Sep 3;297(5):G999–G1008. doi: 10.1152/ajpgi.00204.2009

Fig. 3.

Fig. 3.

IL-8 production and angiogenesis by B.P. CM are NF-κB dependent. A: B.P. CM increased phosphorylation of NF-κB as tested by Western blot analysis. B: HIMECs were subjected to a tube assay and stimulated with B.P. CM along with NF-κB inhibitor SN50 (20 μM) and its negative control peptide SN50M (20 μM) or another NF-κB inhibitor, celastrol (2.2 μM), for 6 h. Inhibitors of NF-κB blocked B.P. CM-induced angiogenesis. Error bars represent SD of triplicate samples. Similar results were observed in 3 independent experiments; *P < 0.05 vs. B.P. CM. C: the result of ELISA for IL-8 showed that B.P. CM-increased IL-8 production was decreased in the presence of the NF-κB inhibitor celastrol. Error bars represent SD of triplicate samples. Similar results were observed in 3 independent experiments; *P < 0.001 vs. B.P. CM.