Fig. 2.

S-CYP cells have constitutively increased reactive oxygen species production but normal levels of HNE. A: relative fluorescent intensity in untreated VEC and S-CYP15 (S-CYP) control cells (Con) and cells treated for 2 h with 85 μM HNE or 3.5 mM H₂O₂ (*P < 0.02; #P < 0.0001 compared with VEC cells with the same treatment). B: levels of HNE-histidine adducts in VEC and S-CYP cells untreated or treated for the indicated number of hours with 85 μM HNE (*P < 0.02 compared with VEC cells with the same treatment). Results are means + SE from 3 independent experiments each with duplicate data points.